Recombinant Murine Granulocyte-Macrophage Colony Stimulating Factor
(rMuGM-CSF)
Catalog Number: C22-122-03
Source: Escherichia coli.
Molecular Weight: Recombinant murine GM-CSF is a 14.2 kDa globular protein consisting of 124 amino acids residues.
Quantity: 5μg/20μg/1000μg
AA Sequence: MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF
EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK
KPVQK
Purity: >95% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
assay usingmurine FDC-P1 cells is less than 0.2 ng/ml, corresponding to a specific activity of＞ 5.0
× 106 IU/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Endotoxin: Less than 1EU/μg of rMuGM-CSF as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
-70°C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further
evaluation purposes. NOT FOR HUMAN USE.
Murine Granulocyte- Macrophage Colony Stimulating Factor
Granulocyte- Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a factor that can support the in
vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and
eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast
cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic
cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and
eosinophils. GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of
autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected
tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core
of four bundled α-helices. Mature mouse GM-CSF shares 49% - 54% amino acid sequence identity with canine, feline, human,
and porcine GM-CSF and 69% with rat GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor
complex composed of GM-CSF Rα/CD116 and the signal transducing common β chain (CD131) which is also a component of
the high-affinity receptors for IL-3 and IL-5. In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF Rα.
The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells,
although rat GM-CSF is fully active on mouse cells.
