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QIAGEN-tip质粒提取试剂盒

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产品名称: QIAGEN-tip质粒提取试剂盒
产品型号: C02-10023
产品展商: QIAGEN
产品价格: 0.00 元
会员价格: 0.00 元
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简单介绍

重力流树脂柱,用于纯化获得*高达20 ug的质粒DNA 相当于两倍氯化铯梯度离心的纯度 可靠的高产量,达到转染级别纯度的质粒DNA 无EB,酚和氯仿,无需氯化铯 经济实惠


QIAGEN-tip质粒提取试剂盒  的详细介绍
QIAGEN Plasmid Mini Kit Specifications
Expected yield*
Column capacity
Culture volume
Up to 20 µg
20 µg
3 –10 ml

Note: For other miniprep spin and vacuum formats, See Plasmid Miniprep Kit Selection guide.
* Actual yields depend on plasmid copy number, size of insert, host strain, culture medium, and culture volume.
Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.

Principle

QIAGEN-tip质粒提取试剂盒The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation (see Figure "Ultrapure Plasmid DNA Yielded by QIAGEN Plasmid kits"). Pre-packed QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.

Ultrapure Plasmid DNA Yielded by QIAGEN Plasmid kits

QIAGEN-tip质粒提取试剂盒 Electron micrograph of pCMVLuc DNA prepared using a QIAGEN-tip 2500. (Data kindly provided by E. Spiess, German Cancer Research Center, Heidelberg, Germany.)

Procedure

With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer (see flowchart). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.

QIAGEN-tip质粒提取试剂盒

Applications

Plasmid DNA purified with QIAGEN Plasmid Kits is ideal for use in applications such as plasmid-mediated gene silencing, transfection (see figure "Transfection Efficiency vs Plasmid Purification Method "), cloning, manual or automated sequencing, including capillary sequencing, and in vitro transcription.

Transfection Efficiency vs Plasmid Purification Method

QIAGEN-tip质粒提取试剂盒 Different pRSVcat DNA preparations using the methods indicated were introduced into the indicated cell lines by liposome-mediated transfection, and the efficiencies determined by measuring CAT expressed after 40 h. Each bar represents the mean of 4 independent transfections (2 transfections with each of 2 independent plasmid preparations).

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