C22-123-01 rMuTNF-α说明书

Catalog Number:  C22-123-01
Source:  Escherichia coli.
Molecular Weight:  Approximately 17.3 kDa. The recombinant murine TNF-α is a soluble 157 amino acid protein which
 corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Quantity:  5μg/20μg/1000μg
AA Sequence:  MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLV
 Y SQVLFKGQGC PDYVLLTHTV SRFAISYQEKVNLLSAVKSP CPKDTPEGAE LKPWYEPIYL
 GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Purity:  >97% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by a cytotoxicity assay
 usingmurine L929 cells is less than 0.1 ng/ml, corresponding to a specific activity of＞ 1.0 × 107
 IU/mg in the presence of the metabolic inhibitor actinomycin D.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered solution in PBS, pH 7.2.
Endotoxin:  Less than 1EU/μg of rMuTNF-α as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Mouse Tumor Necrosis Factor-alpha
Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells,
astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-α occurs as a membrane-anchored
form. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable
biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show
approximately 79% homology at the amino acid level and crossreactivity between the two species.