C22-121-10 rMuIL-10说明书

Catalog Number:  C22-121-10
Source:  Escherichia coli.
Molecular Weight:  Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 161 amino acids.
Quantity:  2μg/10μg/1000μg
AA Sequence:  MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT
 DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK
 TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC
 IEAYMMIKMK S
Purity:  >97% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
 assay usingmurine MC/9-2 cells is less than 2 ng/ml, corresponding to a specific activity of＞ 5.0 × 105 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Endotoxin:  Less than 1EU/μg of rMuIL-10 as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and
 stored at <-20 　C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Murine Interleukin-10
Interleukin-10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages,
monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and
TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10
active on murine cells, but murine IL-10 is inactive on human cells.