人白介素-10|Human IL-10

Synonyms :

B-TCGF, CSIF, TGIF

Description :

IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. Recombinant human IL-10 is an 18.6 kDa protein of 161 amino acid residues.

Catalog #:

200-10

Source :

E.coli

Stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20⁰C. Reconstituted IL-10 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20⁰C. Avoid repeated freeze/thaw cycles.

Purity :

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Biological Activity :

Determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED₅₀ was found to be ≤ 2 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁵ units/mg.

Selected References :

Selected List of References Citing This PeproTech Product

AA Sequence :

MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

PubMed Link :

IL-10

Country of Origin :

USA