重组人 WISP1 / CCN4 蛋白|WISP1 / CCN4 Protein|参数|产品详情

WISP1 / CCN4 蛋白产品信息
产品名称：重组人 WISP1 / CCN4 蛋白|WISP1 / CCN4 Protein|参数|产品详情
WISP1 别名: WISP1, CCN4, WISP1c, WISP1i, WISP1tc
Protein Construction: A DNA sequence encoding the human WISP1 (NP_003873.1 ) (Met 1- Asn 367) was expressed with a C- terminal polyhistidine tag.
种属: Human
表达宿主: Human Cells

WISP1 / CCN4 蛋白质控指标
纯度: > 95%, as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per 1μg of the protein as determined by the LAL method.
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal: Thr 23
分子量: The secreted recombinant human WISP1 consisting of 356 amino acids has a calculated molecular mass of 39 KDa. As a result of glycosylation, It migrates as an approximately 55KDa protein in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS , pH 7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
WISP1 / CCN4 蛋白使用指南
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
WISP1 / CCN4 Protein Description
Human WISP1 (WNT1 inducible signaling pathway protein 1), also named CCN4, is a secreted, heparin-binding glycoprotein, and belongs to the CCN (CTGF/CYR61/NOV) family. Members of the CCN family demonstrate high structural homology sharing four conserved cysteine-rich modular domains: a IGFBP (insulin-like growthfactor-binding) domain, a von Willebrand type C domain, a thrombospondin domain and a C-terminal cysteine -knot domain. WISP1 is a putative downstream effector of the Wnt/Frizzled pathway that mediates diversedevelopmental processes, and also identified as a β-catenin-regulated gene, thus may contributes to Wnt-1-mediated tumorigenesis and malignance. WISP1 is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. It was reported that WISP1 interacts with sulfated glycoconjugates, decorin and biglycan in the ECM of connective tissue, and possibly prevents their inhibitory activity in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage. However, a negatively regulated pathway by WISP1 involving integrins and Rac in the down-regulation of invasion was also illustrated. WISP1 variants lacking certain modules, generated by alternative splicing or gene mutations, have been implicated in various pathological conditions.
参考文献
Pennica, D. et al., 1998, Proc. Natl. Acad. Sci. USA. 95: 14717-14722.
Desnoyers, L. et al., 2001, J. Biol. Chem. 276: 47599-47607.
Xu, L. et al., 2000, Genes Dev. 14: 585-595.
Soon, L.L. et al.,2003, J. Biol. Chem. 278: 11465-11470.
Yanagita, T. et al., 2007, FEBS. J. 274: 1655-1665.
Tanaka, S .et al., 2001, Oncogene. 20: 5525-5532.

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