重组人 EGF / Epidermal Growth Factor 蛋白|EGF蛋白|技术资料|现货

EGF蛋白产品信息
产品名称：重组人 EGF / Epidermal Growth Factor 蛋白|EGF蛋白|技术资料|现货
EGF 别名: HOMG4, URG,  EGF
表达方式: A DNA sequence encoding the mature soluble form of human EGF (NP_001954.2) (Asn 971- Arg 1023) was fused with the Fc region of human IgG1 at the N-terminus.
种属: Human
表达宿主: Human Cells

EGF Protein QC Testing
纯度: >90 % as determined by SDS-PAGE. SDS-PAGE:
生物活性:Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts.
The ED50 for this effect is typically 0.1-0.6 ng/mL.
内**: < 1.0 EU per 1μg of the protein as determined by the LAL method.
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Glu 20
分子量: The recombinant human Fc/EGF chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 290 amino acids and has a calculated molecular mass of 33 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFc/EGF monomer is approximately 37 kDa due to the glycosylation.
缓冲液: Lyophilized from sterile PBS , pH 7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
EGF Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted into smaller quantities for optimal storage and usage; Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EGF蛋白背景综述
Human epidermal growth factor (EGF), also known as urogastrone (URG) since it was isolated from urine based on its inhibitory effect on gastric secretion, is the founding member of the EGF-family of growth factors. All the members of this protein family have highly similar structural and functional characteristics. EGF is initially synthesized as a 130 kDa single-pass transmembrane protein containing 9 EGF-like units and 9 LDL-receptor class B repeats. The mature soluble EGF is a 6 kDa peptide corresponding to the EGF unit located proximal to the transmembrane domain. EGF is expressed in kidney, salivary gland, cerebrum and prostate, and plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. As a potent mitogen, EGF stimulates the growth of various epidermal and epithelial tissues both in vivo and in vitro. Furthermore, EGF is also identified as a magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4), a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion.
参考文献
Ciardiello, F. et al., 1991, Proc. Natl. Acad. Sci. USA. 88: 7792-7796.
Gout, I. et al., 1992, Biochem. J. 288: 395-405.
Harris, R.C. et al., 2003, Exp. Cell. Res. 284: 2-13.
Dreux, A.C. et al., 2006, Atherosclerosis. 186: 38-53.
Groenestege, W.M.T. et al., 2007, J. Clin. Invest. 117: 2260-2267.

包装：200ug

1、产品质量问题，在产品售出7日内提出异议并且产品未有损坏，试剂类产品，需要提供详细的检测数据，创赛负责免费退换货服务；
2、本产品属于消耗品，不提供质保服务；
3、所有产品售后问题，请直接联系我司，我们将会尽快为您响应处理
查询关键词：重组人 EGF / Epidermal Growth Factor 蛋白，EGF蛋白，技术资料，现货，原料药，大包装定制，现货促销，医药中间体，科学仪器，实验室耗材，生化试剂，创赛中国,细胞因子
CANSPEC品质提供的重组人 EGF / Epidermal Growth Factor 蛋白|EGF蛋白|技术资料|现货性能**，其优良的品质满足您不同实验的多种需求。
本产品由上海创赛品质提供，欢迎咨询国内免费服务专线：400-608-7598，更多产品信息请登录www.canspecsci.com或www.4006087598.com