重组人 EGF / Epidermal Growth Factor 蛋白|EGF Protein|技术资料|参数

EGF蛋白产品信息
产品名称：重组人 EGF / Epidermal Growth Factor 蛋白|EGF Protein|技术资料|参数
别名: EGF,   HOMG4,   URG
表达方式: A DNA sequence encoding the mature form of human EGF ( NP_001954.2 ) ( Asn 971 - Arg 1023 ) was expressed and purified, with an initial Met at the N-terminus
种属: Human
表达宿主: E. coli

EGF蛋白质控指标
纯度: > 92 % as determined by SDS-PAGE SDS-PAGE生物活性:Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
( Rubin, J.S. et al. 1991, Proc. Natl. Acad. Sci. USA 88:415. )
The ED50 for this effect is typically 20 - 100 pg/mL
内**: Please contact us for more information.
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Met 1
分子量: The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.3 KDa as estimated in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS , pH 7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
 
EGF蛋白使用指南
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EGF蛋白背景综述
Human epidermal growth factor (EGF) exerts actions on a wide variety of biological processes, and plays an important role in the regulation of cell growth, proliferation, and differentiation mediated by its high-affinity receptor EGFR on the cell surface. It induces differentiation of specific cells, promotes angiogenesis, and inhibits gastric acid secretion in vivo, while serves as a potent mitogenic actor for a variety of cultured cells of both ectodermal and mesodermal origin. Human EGF was originally isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone accordingly. EGF is the founding member of the EGF-family of proteins which are characterized by the presence of at least one EGF structural unit (a conserved 6 cysteine motif that forms three disulfide bonds). The EGF precursor is believed to exist as a membrane-bound molecule containing 9 EGF units, and is proteolytically cleaved to generate the 53-amino acid peptide hormone.
参考文献
Carpenter, G and Cohen, S. 1990. J. Biol. Chem.265: 7709-7712.
Hommel, U. et al., 1992, J. Mol. Biol. 227: 271-282.
Goodsell, D.S. et al., 2003, Stem Cells. 21: 702-703.

包装：50ug  200ug

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