Ephrin-A1蛋白产品信息
产品名称：重组人 EFNA1 / Ephrin-A1 蛋白|Ephrin-A1 ( EFNA1 ) Protein|技术资料|现货
别名: EFNA1 , B61 , ECKLG , EFL1 , EPLG1 , LERK1 , TNFAIP4
表达方式: A DNA sequence encoding the human EphrinA1 ( NP_004419.2 ) without the propeptide ( Met1 - Ser182 ) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus
种属: Human
表达宿主: Human Cells
Ephrin-A1蛋白质控指标
纯度: > 85 % as determined by SDS-PAGE SDS-PAGE
生物活性:Measured by its binding ability in a functional ELISA
Immobilized mouse EphA2 at 2 μg/ml ( 100 μl/well ) can bind human EphrinA1 with a linear range of 0.8 - 20 ng/ml
内**: < 1.0 EU per μg of the protein as determined by the LAL method.
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Asp 19
分子量: The recombinant human EphrinA1 / Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 412 amino acids a predicted molecular mass of 47.5 kDa. As a result of glycosylation, rh EphrinA1/Fc monomer migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions
缓冲液: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
Ephrin-A1蛋白使用指南
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Ephrin-A1蛋白背景综述
The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs. Almost all Eph receptors are expressed during various well defined stages of development in assorted locations and concentrations. EphrinA1, also known as B61, LERK-1, and EFL-1, is a member of the ephrin ligand family which binds members of the Eph receptor family. EphrinA1 has been shown to bind EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. The extracellular domains of human and mouse ephrin-A1 share 85% amino acid identity.
参考文献
Cerretti, DP. et al., 1995, Mol. Immunol. 32 (16): 1197-205.
Gale, NW. et al.,1996, Neuron.17 (1): 9-19.
Pasquale, EB. et al., 1997, Curr. Opin. Cell. Biol. 9 (5): 608-15.
Flanagan, J. et al., 1998, Annual Review of Neuroscience. 21: 309-45.
Holder, N. et al., 1999, Development. 126 (10): 2033-44.
Wilkinson, DG. et al., 2001, Nat. Rev. Neurosci. 2 (3): 155-64.
Murai, KK. et al., 2003, Journal of Cell Science. 116 (Pt 14): 2823-32.
包装：50ug
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