重组人 EphrinA5 蛋白｜Ephrin-A5 Protein|产品详情|报价

Ephrin-A5蛋白产品信息
产品名称：重组人 EphrinA5 蛋白｜Ephrin-A5 Protein|产品详情|报价
Ephrin-A5 别名: EFNA5, AF1, EFL5, RAGS, EPLG7, GLC1M, LERK7
表达方式: A DNA sequence encoding the extracellular domain (Met 1- Asn 203) of human Ephrin-A5 (NP_001953.1) precursor was expressed with a C-terminal polyhistidine tag.
种属: Human
表达宿主: Human Cells
Ephrin-A5蛋白质控指标
纯度: > 90%, as determined by SDS-PAGE SDS-PAGE:
内**: < 1.0 EU per 1μg of the protein as determined by the LAL method.
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Gln 21
分子量: The recombinant human Ephrin-A5 consists of 194 amino acids and has a calculated molecular mass of 23 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphrin-A5 is approximately 27 kDa due to glycosylation.
缓冲液: Lyophilized from sterile PBS , pH 7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

Ephrin-A5蛋白使用指南
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Ephrin-A5蛋白背景综述
Ephrin-A5, also known as AL-1, RAGS, or LERK-7 (ligand for eph-related kinases, 7), is a member of the ephrin ligand family which binds the members of Eph receptor subfamily of tyrosine kinases. Based on their structure and sequence relationships, ephrins are divided into ephrin-A and ephrin-B class, and ephrin-A members are anchored to the membrane by GPI linkage. Ephrin-A5 is expressed with the highest levels in human ***** brain, heart, spleen, and ovary and human fetal brain, lung, and kidney. This ligand has been reported to bind Eph A2, Eph A3, Eph A4, Eph A5, Eph A6, Eph A7, Eph A8, and Eph B2 receptors. The interactions between Eph receptors and their ephrin ligands have been implicated in mediating developmental events, particularly in the nervous system through regulating cell migration and axon pathfinding. It has been reported that Ephrin-A5 prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation.
参考文献
Wilkinson, D.G. 2001, Nat. Rev. Neurosci. 2: 155-164.
Kozlosky, C.J. et al., 1997, Cytokine. 9: 540-549.
Pasquale, E.B. et al., 1997, Curr. Opin.Cell Biol. 9: 608-615.
Himanen, J.P. et al., 2004, Nat. Neurosci. 7: 501-509.
Eberhart, J. et al., 2004, J. Neurosci. 24: 1070-1078.

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