重组小鼠 EFNB1 / Ephrin-B1 蛋白|Mouse EFNB1 / Ephrin-B1 Protein|现货|参数

EFNB1 / Ephrin-B1蛋白产品信息
产品名称：重组小鼠 EFNB1 / Ephrin-B1 蛋白|Mouse EFNB1 / Ephrin-B1 Protein|现货|参数
别名: RP23-110A15.1,Cek5-L, EFL-3, Elk-L, Epl2, Eplg2, LERK-2, Lerk2, Stra1
表达方式: A DNA sequence encoding the mouse EFNB1 ( NP_034240.1 ) extracellular domain ( Met 1 - Ser 229 ) was expressed, fused with a polyhistidine tag at the C-terminus
种属: Mouse
表达宿主: Human Cells
EFNB1 / Ephrin-B1蛋白质控指标
纯度: > 95% as determined by SDS-PAGE SDS-PAGE
生物活性:Measured by its ability to compete with mouse  EphrinB1 / Fc for binding to immobilized mouse EphB3 in a functional ELISA assay
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Lys 30
分子量: The secreted recombinant mouse EFNB1 consists of 211 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNB1 is approximately 33-38 kDa due to glycosylation
缓冲液: Lyophilized from sterile PBS , pH 7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

EFNB1 / Ephrin-B1蛋白使用指南
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EFNB1 / Ephrin-B1蛋白背景综述
The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Mouse ephrin-B1, also known as LERK-2, ELK-L, EFL-3, Cek5-L, and STRA-1, is a member of the ephrin ligand family which binds members of the Eph receptor family. Ephrin-B1 has been shown to bind EphA3, EphB1, EphB2, EphB3, and EphB4. The extracellular domains of human and mouse ephrin-B1 share 94% amino acid identity.
参考文献
Cerretti, DP. et al., 1995, Mol. Immunol. 32 (16): 1197-205.
Gale, NW. et al.,1996, Neuron.17 (1): 9-19.
Flanagan, J. et al., 1998, Annual Review of Neuroscience. 21: 309-45.
Holder, N. et al., 1999,  Development. 126 (10): 2033-44.
Wilkinson, DG. et al., 2001, Nat. Rev. Neurosci. 2 (3): 155-64.
Murai, KK. et al., 2003, Journal of Cell Science. 116 (Pt 14): 2823-32.

包装：5ug  100ug

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