重组人 CXCL4 / PF4 蛋白|Platelet Factor 4 (Chemokine (C-X-C Motif) Ligand 4) Protein

CXCL4 / PF4蛋白产品信息
产品名称：重组人 CXCL4 / PF4 蛋白|Platelet Factor 4 (Chemokine (C-X-C Motif) Ligand 4) Protein
别名: PF4, CXCL4, MGC138298, SCYB4
表达方式: A DNA sequence encoding the human CXCL4 ( NP_002610.1 ) ( Glu 32 - Ser 101 ) was expressed.
种属: Human
表达宿主: E.Coli

CXCL4 / PF4 Protein QC Testing
纯度: > 80 % as determined by SDS-PAGE SDS-PAGE
生物活性:Measured by its ability to inhibit human bFGF / FGF2 dependent proliferation of  Balb/c3T3  mouse fibroblasts
The ED50 for this effect is typically  5-40 μg/mL
Measured by its binding ability in a functional ELISA
Immobilized human CXCL4 at 20 μg/mL ( 100 μl/well ) can bind biotinylated human VEGF165
The EC50 of biotinylated human VEGF165 is  9.97 μg/mL
内**: Please contact us for more information..
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Met
分子量: The recombinant human CXCL4 comprises 71 amino acids with a predicted molecular mass of 7.8 kDa. It migrates as an approximately 11-15 kDa band in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS , pH 7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

CXCL4 / PF4 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and usage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CXCL4 / PF4蛋白背景综述
Platelet factor 4 (PF4) is a 70-amino acid protein belonging to the CXC chemokine family also known as chemokine (C-X-C motif) ligand 4 (CXCL4) . It is released from the alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with a splice variant of the chemokine receptor CXCR3, known as CXCR3B. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 plays a critical role in inflammation and wound repair. PF4 is the antigen in heparin-induced thrombocytopenia, an idiosyncratic autoimmune reaction to the administration of the anticoagulant heparin. Its autoantibodies have been found in patients with thrombosis and features resembling HIT but no prior administration of heparin.
参考文献
Deuel, T.F. et al., 1981, Proc. Natl. Acad. Sci. U.S.A. 78 (7): 4584–4587.
Eisman, R. et al., 1990, Blood. 76 (2): 336–344.
O'Donovan, N. et al.,1999, Cytogenet Cell Genet. 84 (1-2): 39–42.
Lasagni, L. et al.,2003, J Exp Med.197 (11): 1537–1549.
Warkentin, T. E., 2007, N. Engl. J. Med. 356 (9): 891–893.
Warkentin, T. E, et al., 2008, Am. J. Med. 121 (7): 632–636.

包装：5ug  25ug 

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查询关键词：重组人 CXCL4 / PF4 蛋白，Platelet Factor 4 (Chemokine (C-X-C Motif) Ligand 4) Protein，原料药，大包装定制，现货促销，医药中间体，科学仪器，实验室耗材，生化试剂，创赛中国,细胞因子
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