重组小鼠 FCGRT & B2M Heterodimer 蛋白|Mouse FCGRT & B2M Heterodimer Protein|技术资料

FCGRT & B2M Heterodimer蛋白产品信息

产品名称：重组小鼠 FCGRT & B2M Heterodimer 蛋白|Mouse FCGRT & B2M Heterodimer Protein|技术资料
Protein Name : Heterodimer of IgG receptor FcRn large subunit p51 ( alpha chain ) ( FCGRT ) and Beta-2-microglobulin ( beta chian, subunit p14 ) ( B2M )
Type: Mouse FCGRT & B2M Heterodimer Protein
表达方式: A DNA sequence encoding the extracellular domain (Met 1- Ser 297) of mouse FCGRT (Q61559) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887 ) (Met 1- Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified.
种属: Mouse
表达宿主: Human Cells

FCGRT & B2M Heterodimer Protein QC Testing

纯度: > 97 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ser 22 & Ile 21
分子量: The recombinant heterodimer of mouse FCGRT/B2M comprises 386 (287+99) amino acids and has a calculated molecular mass of 44.2 (32.5+11.7) KDa. The apparent molecular mass of rm FCGRT/B2M heterodimer is approximately 48 & 13 KDa respectively in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

FCGRT & B2M Heterodimer Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FCGRT & B2M Heterodimer蛋白背景综述
IgG receptor FcRn large subunit p51, also known as IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor, FCGRT and FCRN, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. FcRn / FCGRT is a MHC class I like molecule that functions to protect IgG and albumin from catabolism. FcRn / FCGRT binds to the Fc region of monomeric immunoglobulins gamma. It mediates the uptake of IgG from milk. It may play a role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin, also known as B2M, is a secreted protein which belongs to the beta-2-microglobulin family. It contains one Ig-like C1-type (immunoglobulin-like) domain. Beta-2 microglobulin (B2M) plays a pivotal role in the biology of mammals. B2M forms the small invariable light chain subunit of class I HLA antigens on the cell membrane of all nucleated cells. During the continuous turnover of the HLA molecules. B2M is shed from the cell membrane into blood. Lymphocytes are the main source of serum free B2M. It associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules. B2M is necessary for cell surface expression of MHC class I and stability of the peptide binding groove. In fact, in the absence of B2M, very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. FcRn complex consist of two subunits: p51, and p14 which is equivalent to beta-2-microglobulin (B2M). It forms an MHC class I-like heterodimer. B2M is a component of the class I major histocompatibility complex (MHC). B2M is also an integral component of the FcRn heterodimer. Failure of passive transfer (FPT) is a condition in which neonates do not acquire protective serum levels of maternal antibodies. A principal component of antibody transport is the neonatal receptor for the Fc portion of immunoglobulin, a heterodimer of a MHC-1 alpha-chain homolog ( FcRn ) and beta-2-microglobulin ( B2M ).
参考文献

He XH. et al., 2004, Sheng Wu Gong Cheng Xue Bao. 20: 99-103.
Clawson ML. et al., 2004, Mamm Genome. 15 (3): 227-36.
Kihara M. et al., 2006, J Biol Chem. 281: 31061-9.
Ricagno S. et al., 2009, Biochem Biophys Res Commun. 380: 543-7.
Kuo TT. et al., 2010, J Clin Immunol. 30 (6): 777-89.

包装：50ug

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