Recombinant PreScission Protease|重组PreScission蛋白酶|保存-20℃|现货|厂家

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产品名称：Recombinant PreScission Protease|重组PreScission蛋白酶|保存-20℃|现货|厂家

Catalog Number: C22-461-03
Source: Escherichia coli.
Description:  PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus
(HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include
the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues.
Substrate recognition and cleavage are likely to be dependent not only upon primary structural
signals, but also upon the secondary and tertiary structures of the fusion protein as well.
Quantity: 100IU/250IU/5000IU
Unit Definition: One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to
90% completion at 5°C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA,
and 1 mM DTT.
Cleavage Buffer:
50mM Tris-HCl, pH7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5°C
prior to use.
Recommended ：Conditions  for Cleavage  of a Fusion 
Protein:During cleavage reactions, it is recommended that samples be removed at various time points and
analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of
PreScission Protease, temperature and length of incubation required for complete digestion of a given
GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion
should be determined in pilot experiments.Digestion may be improved by adding TritonTM X-100,
TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents
up to 1% do not inhibit PreScission Protease.
Storage:  Should be stored in small aliquots at -20°C for long term

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