Recombinant Murine LIF|重组小鼠白血病抑制因子|纯度> 98 % |现货|实验说明

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产品名称：Recombinant Murine LIF|重组小鼠白血病抑制因子|纯度> 98 % |现货|实验说明

Catalog Number: C22-123-07
Source: Escherichia coli.
Molecular Weight: Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
Quantity: 5µg/25µg/1000µg
AA Sequence: SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC
APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID
VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK
QVISVVVQAF
Purity: > 98 % by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The specific activity is determined by inducing
differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of
rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 × 10*8IU/mg, where 50 units is
defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1
ml agar cultures.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN
20.
Endotoxin: Less than 1 EU/g of rMuLIF as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to
-70 °C. Avoid repeated freeze/thaw cycles.

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