SHH / Sonic hedgehog蛋白产品信息
别名: HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
表达方式:
A DNA sequence encoding the amino acid sequence (Cys 198-Ser 462) of human SHH (Q15465), that is Sonic hedgehog protein C-product, was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
种属: Human
表达宿主: Human Cells
SHH / Sonic hedgehog Protein QC Testing
纯度: > 97 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Cys 198
分子量:
The recombinant human SHH( aa 198-462) consists of 276 amino acids and has a predicted molecular mass of 29 kDa. The apparent molecular mass of the protein is approximately 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from a sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
SHH / Sonic hedgehog Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SHH / Sonic hedgehog蛋白背景综述
Sonic HedgeHog, also known as sonic hedgehog protein, belongs to the hedgehog family and is expressed in fetal intestine, liver, lung, and kidney. It cannot be detected in ***** tissues. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog exhibits both floor plate- and motor neuron-inducing activity. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5, triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type.
参考文献
Ericson J. et al., 1997, Cold Spring Harb Symp Quant Biol. 62: 451-66.
Marigo V. et al., 1996, Proc Natl Acad Sci. 93 (18): 9346-51.
Stone DM. et al., 1996, Nature. 384: 129-34.
规格：20ug
关 键 词：重组人 SHH / Sonic hedgehog (aa 198-462) 蛋白, 参数,技术资料，实验室耗材，生化试剂，上海创赛，博飞美科，实验仪器，厂家
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