Mouse Cystatin-F / CST7 / Cystatin-7 Protein|技术资料|产品详情

Cystatin-F / CST7 / Cystatin-7蛋白产品信息

别名: CST7
表达方式: A DNA sequence encoding the mouse CST7 (O89098) (Met1- Gln144) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
种属: Mouse
表达宿主: Human Cells

Cystatin-F / CST7 / Cystatin-7 Protein QC Testing

纯度: > 93 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ala 19
分子量: The recombinant mouse CST7 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 367 amino acids and has a predicted molecular mass of 41.4 KDa. The apparent molecular mass of the protein is approximately 42 KDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile 25mMTris,0.15MNaCl,pH7.5
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

Cystatin-F / CST7 / Cystatin-7 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Cystatin-F / CST7 / Cystatin-7蛋白背景综述

The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.

参考文献

Ni J. et al., 1998, J Biol Chem. 273: 24797-804.
Morita M. et al., 1999, Cancer Res. 59:151–8.
Langerholc T. et al., 2005, FEBS J. 272: 1535-45.
Hamilton G. et al., 2008, EMBO J. 27: 499-508.
Alexander W. et al., 2006, J Biol Chem. 281: 16570-5.

规格：10ug

关 键 词：Mouse Cystatin-F / CST7 / Cystatin-7 Protein,技术资料,产品详情，实验室耗材，生化试剂，上海创赛，博飞美科，实验仪器，厂家

CANSPEC品质提供的Mouse Cystatin-F / CST7 / Cystatin-7 Protein|技术资料|产品详情性能**，其优良的品质满足您不同实验的多种需求。

本产品由上海创赛品质提供，欢迎咨询国内免费服务专线：400-608-7598，更多产品信息请登录www.canspecsci.com 或 www.4006087598.com