Rat IL4R / Il4ra Protein|技术资料|价格

IL4R / Il4ra蛋白产品信息

别名: IL4R, Il4ra
表达方式: 
A DNA sequence encoding the rat IL4R(Q63257-1)(Met1-Arg232) was expressed, fused with a polyhistidine tag at the C-terminus.
种属: Rat
表达宿主: Human Cells

IL4R / Il4ra Protein QC Testing

纯度: >90% as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ile 26
分子量: 
The recombinant rat IL4R  is a disulfide-linked homodimer. The reduced monomer comprises 218 amino acids and predicts a molecular mass of 25.3 kDa. The apparent molecular mass of  the recombinant protein is approximately 46 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

IL4R / Il4ra Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL4R / Il4ra蛋白背景综述

IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132). In non-hematopoietic cells, the IL4Rα subunit associates with the IL13Rα chain and also serves as a functional complex for IL-13. IL4Rα is a single-pass type I  transmembrane protein containing ser/Pro-rich regions in cytoplasmic domain similar to those present in the IL-2 receptor and GM-CSF receptor ß-chains. As a receptor for both IL4 and IL13, IL4Rα couples to the JAK1/2/3-STAT6 pathway, and is involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation, as well as promoting differentiation of Th2 cells. Two transcript variants encoding different isoforms of IL4Rα, a membrane-bound and a soluble form, have been found, and the soluble form of IL4Rα can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.

参考文献

Grabstein K. et al.,1986, J Exp Med. 163: 1405-14.
Yokota T. et al., 1988, Immunol Rev. 102: 137-87.
Kotowicz K. et al., 1996, Int Immunol. 8: 1915-25.
Murata T. et al., 1998, Int J Mol Med. 1: 551-7.
Nelms K. et al., 1999, Ann Rev Immunol. 17: 701-38.
Hackstein H. et al.,1999, Tissue Antigens. 54 (5): 471-7.
Gessner A. et al., 2000, Immunobiology. 201 (3-4): 285-307.

规格：50ug

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