EPHA4蛋白产品信息
别名: EPHA4
表达方式:
A DNA sequence encoding the Cynomolgus EPHA4 (Met1-Thr547) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
种属: Cynomolgus
表达宿主: Human Cells
EPHA4 Protein QC Testing
纯度: > 96 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Val 20
分子量:
The recombinant Cynomolgus EPHA4 is a disulfide-linked homodimer. The reduced monomer comprises 769 amino acids and has a calculated molecular mass of 85.4KDa.The apparent molecular mass of Cynomolgus EPHA4 heterodimer is approximately 95 KDa respectively in SDS-PAGE.
缓冲液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
EPHA4 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EPHA4蛋白背景综述
Ephrin type-A receptor 4, also known as Tyrosine-protein kinase receptor SEK, Tyrosine-protein kinase TYRO1, EPH-like kinase 8, and EPHA4, is a single-pass type I membrane protein which belongs to the protein kinase superfamily and ephrin receptor subfamily. EPHA4 contains two fibronectin type-III domains, one protein kinase domain and one SAM (sterile alpha motif) domain. EPHA4 is a receptor for members of the ephrin-A family. EPHA4 binds to ephrin-A1, ephrin-A4 and ephrin-A5. It binds more poorly to ephrin-A2 and ephrin-A3. EPHA4 may play a role in a signal transduction process involved in hindbrain pattern formation.
The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosyl- phosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs.
参考文献
Flanagan J. et al., 1998,?Annual Review of Neuroscience.?21: 309-45.
Holder N. et al., 1999,? Development. 126?(10): 2033-44.
Wilkinson DG. et al., 2001,?Nat. Rev. Neurosci.?2?(3): 155-64.
Murai KK. et al., 2003,?Journal of Cell Science.?116?(14): 2823-32.
The MGC Project Team. 2004, Genome Res. 14: 2121-7.
Rikova K. et al., 2007, Cell 131: 1190-203.
Heibeck TH. et al., 2009, J. Proteome Res. 8:3852-61.
规格：50ug
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