Mouse EPO / Erythropoietin Protein|技术资料|参数

EPO / Erythropoietin蛋白产品信息

别名: EPO
表达方式: 
A DNA sequence encoding the mouse EPO (P07321) (Met1- Arg192) was expressed with a C-terminal polyhistidine tag.
种属: Mouse
表达宿主: Human Cells

EPO / Erythropoietin Protein QC Testing

纯度: >96% as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ala 27
分子量: 
The recombinant mouse EPO comprises 177 amino acids and has a predicted molecular mass of 20 kDa. The apparent molecular mass of the protein is approximately 37 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

EPO / Erythropoietin Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EPO / Erythropoietin蛋白背景综述

Erythropoietin, also known as EPO, is a secreted protein which belongs to the EPO / TPO family. Erythropoietin / EPO is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Erythropoietin / EPO is produced by kidney or liver of ***** mammals and by liver of fetal or neonatal mammals. EPO is produced mainly by peritubular fibroblasts of the renal cortex. It is synthesized by renal peritubular cells in *****s, with a small amount being produced in the liver. Erythropoietin / EPO is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Erythropoietin / EPO is the hormone that regulates red blood cell production. It plays an important role in the brain's response to neuronal injury. Erythropoietin / EPO is also involved in the wound healing process. Regulation is believed to rely on a feed-back mechanism measuring blood oxygenation. Erythropoietin / EPO binds to the erythropoietin receptor (EPOR) on the red cell surface and activates a JAK2 cascade. EPOR is also found in a large number of tissues such as bone marrow cells and peripheral/central nerve cells, many of which activate intracellular biological pathways upon binding with EPO. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus.

参考文献

Lin FK. et al., 1985, Proc Natl Acad Sci. 82 (22): 7580-4.
Fisher JW. et al.,1996, British journal of haematology. 95 (1): 27-32.
Macdougall IC. et al., 2000, Semin Nephrol. 20 (4): 375-81.
Siren AL. et al., 2001. Proc Natl Acad Sci. 98 (7): 4044-9.
Haroon ZA. et al.,2003, Am J Pathol. 163 (3): 993-1000.
Jelkmann W. et al., 2007, Eur J Haematol.78 (3): 183-205.

规格：10ug

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