Canine CAIX / CA9 Protein|实验说明|厂家价格

CAIX / CA9蛋白产品信息

别名: CA9
表达方式: 
A DNA sequence encoding the canine CA9 (Met1-Leu410) was expressed with a C-terminal polyhistidine tag.
种属: Canine
表达宿主: Human Cells

CAIX / CA9 Protein QC Testing

纯度: >85% as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Gln 38
分子量: 
The recombinant canine CA9 comprises 384 amino acids and has a predicted molecular mass of 42.1 kDa. The apparent molecular mass of the protein is approximately 50 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

CAIX / CA9 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CAIX / CA9蛋白背景综述

Carbonic anhydrases IX (CAIX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide ( H2O + CO2 = H+ + HCO3- ) and thus participate in a variety of biological and physical processes. CAIX is a transmembrane protein structurally consisting of a signal peptide, a proteoglycan-related region, a CA domain with a highly conserved active site, a transmembrane anchor and an intracytoplasmic tail, and is the only tumor-associated CA isoenzyme known so far. Compared with normal tissues, CAIX is overexpressed in a wide spectrum of tumor types and associated with increased metastasis and poor prognosis in aggressive carcinomas. CAIX expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. CA9 is regarded as a new therapeutic target for CA9-derived carcinomas.

参考文献

Pastorek J. et al., 1994, Oncogene. 9: 2877-88.
Opavsky R. et al., 1996, Genomics. 33: 480-7.
Swietach P. et al., 2008, J Biol Chem. 283: 20473-83.
Robertson N. et al., 2004, Cancer Res. 64: 6160-5.
Bui MH. et al., 2003, Clin Cancer Res. 9: 802-11.
Choi SW. et al., 2008, Hum Pathol. 39: 1317-22.

规格：10ug

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