Influenza A Virus H5N1 (A/Cambodia/S1211394/2008) HA Protein|产品详情|厂家

H5N1 (A/Cambodia/S1211394/2008) HA蛋白产品信息

别名: HA
表达方式: 
A DNA sequence encoding the N-terminal segment (Met 1-Gly 341) of the influenza hemagglutinin (A/Cambodia/S1211394/2008(H5N1)) (ADM95445.1), termed as HA, was fused with a polyhistidine tag at the C-terminus.
种属: Influenza A Virus
表达宿主: Human Cells

H5N1 (A/Cambodia/S1211394/2008) HA Protein QC Testing

纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Asp 17
分子量: 
The secreted recombinant HA1 subunit of influenza A H5N1 HA (A/Cambodia/S1211394/2008(H5N1)) comprises 526 amino acids and has a predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 83 kDa band in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile  PBS-2, pH7.4, 10%gly.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

H5N1 (A/Cambodia/S1211394/2008) HA Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

H5N1 (A/Cambodia/S1211394/2008) HA蛋白背景综述

Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion "fusion peptide". After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle's own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

参考文献

Barman S. et al., 2000, J Virol. 74: 6538-45.
Suzuki T. et al., 2005, J Virol. 79: 11705-15.
Shinya K. et al., 2006, Nature. 440 (7083): 435-6.
Marjuki H. et al., 2006, J Biol Chem. 281: 16707-15.
Christophe F. et al., 2009, Science. 324: 1557-61.
Von Itzstein M. 2007, Nat Rev Drug Discov. 6: 967-74.

规格：50ug

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