IFNA4 / IFNα4 / Interferon alpha-4蛋白产品信息
别名: IFNA4
表达方式:
A DNA sequence encoding the rat IFNA4 (D3ZFH0)(Cys24-Lys189) was expressed, fused with Fc region of human IgG1 at the N-terminus.
种属: Rat
表达宿主: Human Cells
IFNA4 / IFNα4 / Interferon alpha-4 Protein QC Testing
纯度: > 90 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Glu
分子量:
The recombinant rat IFNA4 comprises 426 amino acids and predicts a molecular mass of 47.5 kDa. The apparent molecular mass of the recombinant protein is approximately 48 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
IFNA4 / IFNα4 / Interferon alpha-4 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IFNA4 / IFNα4 / Interferon alpha-4蛋白背景综述
Interferons (IFNs) are natural glycoproteins belonging to the cytokine superfamily, and are produced by a wide variety of cells types in response to challenges by foreign agents such as viruses, parasites and tumor cells. There are three major classes of IFNs according to the receptor type through which they signal, and the type I IFNs bind to a specific receptor complex known as the IFN-α receptor (IFNAR) consisting of IFNAR1 and IFNAR2 chains. The type I IFNs present in humans are IFN-α, IFN-β and IFN-ω, and the IFN-α come in 13 subtypes whose encoding genes locate in a cluster on chromosome 9. IFN-α is produced by leukocytes and mainly involved in innate immune response against viral infection together with other IFNs. IFN-α is produced in response to the presence of dsRNA, a key indicator of viral infection via Toll Like Receptors, and assists the immune response by inhibiting viral replication within host cells, activating natural killer cells and macrophages, increasing antigen presentation to lymphocytes, and inducing the resistance of host cells to viral infection. IFN-α also acts as a pyrogenic factor and thus causes fever by binding to opioid receptors and eliciting the release of prostaglandin-E2, and serves as an analgesic by employing the similar mechanism. Furthermore, IFN-α is suggested to promote apoptosis and accordingly has therapeutic effect against certain cancers by inducing increased production of the p53 gene product. Here, the IFN-α4a is an isoform of the human IFNA4 subtype.
参考文献
Morikawa K. et al., 1987, J Immunol. 139: 761-6.
Sato K. et al., 2001, Eur J Immunol. 31: 3146-88.
Takaoka A. et al., 2003, Nature. 424: 516-23.
Hardy. et al., 2004, Genomics. 84: 331-45.
Pirhonen J. et al., 2007, J Leukoc Biol. 82: 1185-92.
Badr G. et al., 2005, Int Immunol. 17: 459-67.
Hussain M. et al., 1997, J Interferon Cytokine Res. 17: 559-66.
规格：20ug
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