TNFRSF1A / TNFR1蛋白产品信息
别名: TNFRSF1A, Tnfr-1, Tnfr1
表达方式:
A DNA sequence encoding the rat TNFRSF1A (P22934)(Met1-Ala211) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
种属: Rat
表达宿主: Human Cells
TNFRSF1A / TNFR1 Protein QC Testing
纯度: > 90% as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ile 22
分子量:
The recombinant rat TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 431 amino acids and has a predicted molecular mass of 47.9 kDa. The apparent molecular mass of the protein is approximately 59 kDa in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
TNFRSF1A / TNFR1 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
TNFRSF1A / TNFR1蛋白背景综述
Tumor necrosis factor receptor superfamily, member 1A (TNFRSF1A), also known as TNFRI, TNF R-p55/p60, and CD120a, is a type I transmembrane protein widely expressed and is present on the cell surface as a trimer of 55 kDa subunits. Human TNFRSF1A/TNFRI is a 455 aa protein that contains a 21 aa signal sequence, a 190 aa ECD with a PLAD domain which mediates constitutive trimer formation followed by four TNF-α trimer-binding cysteine-rich domains (CRDs), a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain. TNFRSF1A/TNFRI serves as a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor, and the resulting death-inducing signaling complex (DISC) initiates the subsequent cascade of caspases mediating apoptosis. TNFRSF1A/TNFRI can also activate the transcription factor NF-kB, and functions as a regulator of inflammation. Germline mutations of the extracellular domains of TNF RI are found to be associated with the human genetic disorder called periodic fever syndrome.
参考文献
Lewis M. et al., 1991, Proc Natl Acad Sci. 88: 2830-4.
Banner DW. et al., 1993, Cell. 73: 431-45.
Medvedev AE. et al., 1996, J Biol Chem. 271: 9778-84.
Peschon JJ. et al., 1998, J Immunol. 160: 943-52.
Chan FK. et al., 2000, Science. 288: 2351-4.
Micheau O. et al., 2003, Cell. 114 (2): 181-90.
Hehlgans T. et al., 2005, Immunology. 115: 1-20.
规格：50ug
关 键 词：Rat TNFRSF1A / TNFR1 Protein,技术资料,价格，实验室耗材，生化试剂，上海创赛，博飞美科，实验仪器，厂家
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