CD3E蛋白质|CD3E Protein |价格|厂家

CD3E蛋白产品信息

别名: CD3E
表达方式: 
A DNA sequence encoding the human CD3E ( Met 1-Asp126) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
种属: Human
表达宿主: Human Cells

CD3E Protein QC Testing

纯度: (84.6+ 14.1) % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Gln 22
分子量: 
The secreted recombinant human CD3E is a disulfide-linked homodimeric protein. The reduced monomer comprises 355 amino acids and has a predicted molecular mass of 40 kDa. As a result of glycosylation, it migrates as an approximately 44-53 and 38 kDa band in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

CD3E Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD3E蛋白背景综述

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I  membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. This gene has also been linked to a susceptibility to type I  diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

参考文献

Wong S. et al., 1991, Clin Exp Immunol. 83 (1): 69-73.
Call ME. et al., 2004, EMBO J. 23 (12): 2348-57.
Arnett KL. et al., 2004, Proc Natl Acad Sci. 101: 16268-73.
Kjer-Nielsen L. et al., 2004, Proc Natl Acad Sci. 101: 7675-80.
Fischer A. et al., 2005, Curr Opin Allergy Clin Immunol. 5 (6): 491-5.
Holst J. et al., 2008, Nature immunology. 9 (6): 658-66.

规格：20ug

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