E-cadherin / CDH1 / Cadherin-1蛋白产品信息
别名: CDH1
表达方式:
A DNA sequence encoding the rat CDH1 (Q9R0T4) (Met1-Ala713) was expressed with the Fc region of human IgG1 at the C-terminus.
种属: Rat
表达宿主: Human Cells
E-cadherin / CDH1 / Cadherin-1 Protein QC Testing
纯度: > 75 % as determined by SDS-PAGE SDS-PAGE:
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Gln 24
分子量:
The recombinant rat CDH1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 931 amino acids and has a predicted molecular mass of 103.8 kDa. The apparent molecular mass of the protein is approximately 116 kDa in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
E-cadherin / CDH1 / Cadherin-1 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
E-cadherin / CDH1 / Cadherin-1蛋白背景综述
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is the classical cadherin molecule and contains five cadherin domains of ~110 aa each in the extracellular domain. E-Cad is expressed in the non-neural epithelial tissues as disulfide-linked homodimer, and plays a key role in the organization and integrity of most epithelial tissues, cell differentiation and tissue development. CDH1 interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas (gastric, breast, ovary, endometrium and thyroid).
参考文献
Berx G. et al., 1995, Genomics 26: 281-9.
Yoshiura K. et al., 1995, Proc. Natl. Acad. Sci. 92: 7416-9.
Becker K-F. et al., 1994, Cancer Res. 54: 3845-52.
Marambaud P. et al., 2002, EMBO J. 21: 1948-56.
Steinhusen U. et al., 2001, J Biol Chem. 276: 4972-80.
规格：50ug
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