Rat CD40LG / CD40L / TNFSF5 Protein |现货|价格

CD40LG / CD40L / TNFSF5蛋白产品信息

别名: CD40LG, Cd40l, Tnfsf5
表达方式: 
A DNA sequence encoding the rat CD40LG (Gly115-Leu260) was expressed with Fc region of human IgG1 at the N-terminus.
种属: Rat
表达宿主: Human Cells

CD40LG / CD40L / TNFSF5 Protein QC Testing

纯度: > 80 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Glu
分子量: 
The recombinant rat CD40LG comprises 406 amino acids and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of  the recombinant protein is approximately 53 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

CD40LG / CD40L / TNFSF5 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD40LG / CD40L / TNFSF5蛋白背景综述

CD40 ligand, also known as CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, CD154, CD40L and TNFSF5, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. CD40L ( CD154 / TNFSF5 ) is specifically expressed on activated CD4+ T-lymphocytes. The soluble form of CD40L ( CD154 / TNFSF5 ) is derived from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. CD40L ( CD154 / TNFSF5 ) mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. It is involved in immunoglobulin class switching. Defects in CD40L are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1), also known as X-linked hyper IgM syndrome (XHIM). It is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes.

参考文献

Korthaeuer U. et al., 1993, Nature. 361: 539-41.
Lin Q. et al., 1996, J Clin Invest. 97: 196-201.
Seyama K. et al.,1998, Blood. 92: 2421-34.
Khandekar SS. et al., 2001, Protein Expr Purif. 23: 301-10.
Furman MI. et al., 2004, J Am Coll Cardiol. 43: 2319-25.

规格：20ug

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