Rat Cadherin-5 / CDH5 / VE-Cad Protein|价格|参数

Cadherin-5 / CDH5 / VE-Cad蛋白产品信息

别名: CDH5
表达方式: 
A DNA sequence encoding the rat CDH5 (Met1-Gln585) was expressed with a polyhistidine tag at the C-terminus.
种属: Rat
表达宿主: Human Cells

Cadherin-5 / CDH5 / VE-Cad Protein QC Testing

纯度: > 70 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Gly 25
分子量: 
The recombinant rat CDH5 comprises 572 amino acids and predicts a molecular mass of 65.1 kDa. The apparent molecular mass of  the recombinant protein is approximately 91 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

Cadherin-5 / CDH5 / VE-Cad Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Cadherin-5 / CDH5 / VE-Cad蛋白背景综述

Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. They play important roles in cell adhesion, ensuring that cells within tissues are bound together. They are dependent on calcium(Ca2+) ions to function. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I  cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, is single-pass type I  membrane protein. It is expressed in endothelial tissues and brain and is involved in loss of heterozygosity events in breast and prostate cancer. CDH5 is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, CDH5 may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.

参考文献

Angst B. et al., 2001, J Cell Sci. 114 (4): 629-41.
Duguay D. et al., 2003, Dev Biol. 253: 309-23.
Bayas MV. et al., 2005, Biophysical Journal. 90 (4): 1385.
Crosby CV, et al., 2005, Blood. 105 (7): 2771-6.
Hulpiau P. et al., 2009, Int J Biochem Cell Biol. 41 (2): 349-69.

规格：50ug

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