Rat ART4 Protein|鼠ART4蛋白质|价格|参数

ART4蛋白产品信息

别名: ART4
表达方式: A DNA sequence encoding the rat ART4(Met1-Lys269) was expressed with a polyhistidine tag at the C-terminus.
种属: Rat
表达宿主: Human Cells

ART4 Protein QC Testing

纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Gly 30
分子量: 
The recombinant rat ART4 comprises 251 amino acids and predicts a molecular mass of 28.4 kDa. The apparent molecular mass of  the recombinant protein is approximately 36-41 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

ART4 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ART4蛋白背景综述

ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, belongs to a family of ectoenzymes named toxin-related ADP-ribosyltransferases (ARTs) that catalyze the transfer of ADP-ribose from NAD+ to a target protein. ART4 contains a mono-ADP-ribosylation motif. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane.

参考文献

Parusel I. et al., 2005, Cell Immunol. 236 (1-2): 59-65.
Friedrich M. et al., 2005, DNA Seq. 16 (1): 53-7.
Okazaki IJ. et al., 1998, J Biol Chem. 273 (37): 23617-20.

规格：20ug

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