Cadherin-8 / CDH8蛋白产品信息
别名: CDH8
表达方式:
A DNA sequence encoding the rat CDH8 (NP_445845.2)(Met1-Met621) was expressed with a polyhistidine tag at the C-terminus.
种属: Rat
表达宿主: Human Cells
Cadherin-8 / CDH8 Protein QC Testing
纯度: > 85 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ala 30
分子量:
The recombinant rat CDH8 comprises 603 amino acids and predicts a molecular mass of 66.5 kDa. The apparent molecular mass of the recombinant protein is approximately 75-85 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
Cadherin-8 / CDH8 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Cadherin-8 / CDH8蛋白背景综述
Cadherins are integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Type I cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of five subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherin 8, also known as CDH 8, is a type I I classical cadherin belonging to the cadherin superfamily. As mainly expressed in brain, CDH8 is found in certain nerve cell lines, such as retinoblasts, glioma cells and neuroblasts, and is putatively involved in synaptic adhesion, axon outgrowth and guidance. Human Cadherin 8 is a 799 amino acid single-pass type I transmembrane protein with a putative 29 aa signal sequence, and a 32 aa propeptide, a 560 aa mature extracellular domain, a 21 aa transmembrane domain and a 157 aa cytoplasmic domain. The human, mouse and rat proteins share approximately 98% homology.
参考文献
Tanihara H. et al., 1994, Cell Adhes Comm. 2: 15-26.
Kido M. et al., 1998, Genomics. 48: 186-94.
Yamagata K. et al., 1999, J Biol Chem. 274: 19473-9.
Nollet F. et al., 2000, J Mol Biol. 299: 551-72.
Blaschke S. et al., 2002, Int J Cancer. 101 (4): 327-34.
Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.
规格：5ug
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