Cynomolgus NT5E / CD73 Protein|现货|产品详情

NT5E / CD73蛋白产品信息

别名: NT5E
表达方式: 
A DNA sequence encoding the cynomolgus NT5E (Met1-Lys547) was expressed with a polyhistidine tag at the C-terminus.
种属: Cynomolgus
表达宿主: Human Cells

NT5E / CD73 Protein QC Testing

纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Trp 27
分子量: 
The recombinant cynomolgus NT5E comprises 532 amino acids and has a calculated molecular mass of 59.2 KDa. The apparent molecular mass of it is approximately 59 KDa respectively in SDS-PAGE.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

NT5E / CD73 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NT5E / CD73蛋白背景综述

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

参考文献

Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.

规格：10ug

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