CD200蛋白产品信息
别名: CD200
表达方式:
A DNA sequence encoding the cynomolgus CD200 (NP_001244473.1) (Met1-Gly232) was expressed with the Fc region of human IgG1 at the C-terminus.
种属: Cynomolgus
表达宿主: Human Cells
CD200 Protein QC Testing
纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Gln 31
分子量:
The recombinant cynomolgus CD200 is a disulfide-linked homodimer. The reduced monomer comprises 443 amino acids and has a calculated molecular mass of 49.6 KDa. The apparent molecular mass of the protein is approximately 53-57 KDa respectively in SDS-PAGE.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
CD200 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD200蛋白背景综述
CD200, also known as OX-2, is a transmembrane immunoregulatory protein belonging to the immunoglobulin superfamily. The mouse CD200 cDNA encodes a 278 aa precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain, and shares 76% and 94% aa sequence identity with human and rat CD200. CD200, a type 2 transmembrane molecule, plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion by delivering an immunoregulatory signal through interaction with its receptor. CD200 was found to be co-expressed with stem cell markers found on prostate, breast, brain, and colon cancers. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. CD200 and CD200R associate via their respective N-terminal Ig-like domains. In myeloid cells, CD200R initiates inhibitory signals following receptor-ligand contact. In T cells, CD200 functions as a costimulatory molecule independent of the CD28 pathway. CD200 and its interaction with the CD200 receptor is a potential therapeutic target for metastatic melanoma (MM).
参考文献
Chen Z. et al., 1997, Biochim Biophys Acta. 1362 (1): 6-10.
Gorczynski RM. et al., 2001, Clin Exp Immunol. 126 (2): 220-9.
Shiratori I. et al., 2005, J Immunol. 175 (7): 4441-9.
Kawasaki BT. et al., 2007, Biochem Biophys Res Commun. 364 (4): 778-82.
Kretz-Rommel A. et al., 2008, J Immunol. 180 (2): 699-705.
Chen Z. et al., 2009, Mol Immunol. 46 (10): 1951-63.
规格：50ug
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