EPHB2 / Hek5蛋白产品信息
别名: EPHB2, CAPB, DRT, EPHT3, ERK, Hek5, PCBC, Tyro5
表达方式:
A DNA sequence encoding the human EPHB2 (NP_059145.2) (Met 1-Leu 543) was expressed with six amino acids (ENLYFQ ) at the C- terminus.
种属: Human
表达宿主: Human Cells
EPHB2 / Hek5 Protein QC Testing
纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Val 19
分子量:
The recombinant human EPHB2 consists of 532 amino acids and predicts a molecular mass of 59 KDa. It migrates as an approximately 66 KDa band in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
EPHB2 / Hek5 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EPHB2 / Hek5蛋白背景综述
EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a member of the transmembrane Eph receptor tyrosine kinase family (RTKs). Ephrins are divided into the ephrin-A class, anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B class, the transmembrane proteins. The Eph receptor family are divided into two groups based on the similarity of their extracellular domain sequences and affinities for binding ephrin-A and ephrin-B ligands, making up the largest subgroup of the receptor tyrosine kinase (RTK) family. Human EphB2 cDNA encodes a 1055 aa precursor, which includes an 18 aa signal sequence, a 525 aa extracellar domain (ECD) containing a cysteine rich region followed by two fibronectin type I?II domains, a 21 aa transmembrane segment, and a 491 aa cytoplamic domain containing the tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif. It is expressed on both sides of the neuronal synapse controlling axon guidance across the embryonic midline, promoting a neuronal fate from neural precursors, and regulating NMDA receptor activity. EphB2 is a receptor for members of the ephrin-B family, and is proposed as a tumor suppressor. It is indicated that aberrant EphB2 expression and activity are implicated in the progression of several cancers.
参考文献
Fox GM. et al., 1995, Oncogene. 10: 897-905.
Tang XX. et al., 1998, Oncogene. 17: 521-6.
Cowan CA. et al., 2000, Neuron 26: 417-430.
Henderson JT. et al., 2001, Neuron. 32:1041-56.
Katakowski M. et al., 2005, Neurosci Lett. 385: 204-9.
Cramer KS. et al., 2006, Dev Biol. 295: 76-89.
Pasquale EB. 2008, Cell. 133: 38-52.
Merlos-Suarez A. et al., 2008, Curr Opin Cell Biol. 20: 194-200.
规格:50ug
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