Rat CD10 / MME / Neprilysin Protein|技术资料|价格

CD10 / MME / Neprilysin蛋白产品信息

别名: MME
表达方式: 
A DNA sequence encoding the rat MME (P07861) (Tyr52-Trp750) was expressed with a polyhistidine tag at the N-terminus.
种属: Rat
表达宿主: Human Cells

CD10 / MME / Neprilysin Protein QC Testing

纯度: >95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: His
分子量: 
The recombinant rat MME comprises 719 amino acids and has a predicted molecular mass of 82.5 kDa. The apparent molecular mass of the protein is approximately 93 kDa in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

CD10 / MME / Neprilysin Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD10 / MME / Neprilysin蛋白背景综述

Neprilysin, also known as neutral endopeptidase ( NEP ) and MME, is a type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands, and lung. This zinc-dependent metalloprotease enzyme cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. Neprilysin is thought to be the rate-limiting degrading enzyme of amyloid β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer‘s disease (AD). MME is also identified as the common acute lymphoblastic leukemia antigen ( CALLA ) or CD10 present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL). In addition, associations have been observed between neprilysin expression and various types of cancer, and in some cancer models NEP is demonstrated to negatively regulate angiogenesis through proteolytically inactivating the fibroblast growth factor-2 ( FGF-2 ). Accordingly, Loss or a decrease in neprilysin expression has been reported in a several malignancies, as well as Alzheimer's-like behavioral impairment and amyloid-beta deposition in the brain.

参考文献

Shipp MA. et al. 1989, Proc Natl Acad Sci. 86: 297-301.
Goodman OB. et al., 2006, J Biol Chem. 281: 33597-605.
Cohen AJ. 1996, Cancer Res. 56: 831-9.
Iwata N. et al., 2001, Science. 292: 1550-2.
EI-Amouri SS. et al., 2008, Am J Pathol. 172: 1342-54.

规格：10ug

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