EPHA2蛋白产品信息
别名: EPHA2, ECK
表达方式:
A DNA sequence encoding the human EPHA2 (P29317) (Met1-Asn534) was expressed with a polyhistidine tag at the C-terminus.
种属: Human
表达宿主: Human Cells
EPHA2 Protein QC Testing
纯度: > 95% as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ala 24
分子量:
The recombinant human EPHA2 consists of 522 amino acids and predicts a molecular mass of 57.6 KDa. It migrates as an approximately 65-75 KDa band in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
EPHA2 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EPHA2蛋白背景综述
Ephrin type-A receptor 2, also known as Epithelial cell kinase, Tyrosine-protein kinase receptor ECK and EPHA2, is a single-pass type I membrane protein which belongs to the protein kinase superfamily, tyr protein kinase family and ephrin receptor subfamily. EPHA2 is a receptor for members of the ephrin-A family. EPHA2 binds to ephrin-A1, ephrin-A3, ephrin-A4 and ephrin-A5. It plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2. The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosyl- phosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs.
参考文献
Nowakowski J. et al., 2002, Structure. 10: 1659-67.
Murai KK. et al., 2003, Journal of Cell Science. 116 (14): 2823-32.
The MGC Project Team. 2004, Genome Res. 14: 2121-7.
Rikova K. et al., 2007, Cell. 131: 1190-203.
Zhang G. et al., 2008, Cancer Res. 68: 1691-6.
Himanen JP. et al., 2009, EMBO Rep. 10: 722-8.
Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.
规格:100ug
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