TrkA / NTRK1蛋白产品信息
别名: NTRK1, Trk, Trka
表达方式:
A DNA sequence encoding the rat NTRK1 (P35739-Isoform TrkA-II)(Met1-Pro418) was expressed with the Fc region of human IgG1 at the C-terminus.
种属: Rat
表达宿主: Human Cells
TrkA / NTRK1 Protein QC Testing
纯度: > 95% as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ala 33
分子量:
The recombinant rat NTRK1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 627 amino acids and has a predicted molecular mass of 69.2 kDa. The apparent molecular mass of the protein is approximately 92-102 kDa in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
TrkA / NTRK1 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
TrkA / NTRK1蛋白背景综述
Neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), also well known as TrkA, is a member of the neurotrophic tyrosine kinase receptor family belonging to Tyr protein kinase superfamily, which also includes TrkB and TrkC. TrkA/NTRK1 has a complex subdomain orgnazation conserved in the family members, containing two cysteine-rich domains, a cluster of three leucine-rich motifs (LRR), and two Ig-like C2-type domains in the extracellular region, as well as an tyrosine kinase domain in the cytoplasmic region. TrkA/NTRK1 exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures, and is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA/NTRK1 plays an important role in cell survival, differentiation, and apoptosis in various neuronal and nonneuronal cell types, especially in the nervous system by recruiting signaling molecules including SHC1, PI 3-kinase, and PLC-gamma-1 that activate pathways required for the growth and survival of neurons. It has been shown that TrkA/NTRK1 is specially involved in the development and function of the nociceptive reception system and establishment of thermal regulation via sweating. Two distinct TrkA/NTRK1 isoforms have been identified: TrkA-II is primarily expressed in neuronal cells, while isoform TrkA-I is found in non-neuronal tissues. Defects in TrkA/NTRK1 are a cause of a rare autosomal recessive disorder, congenital insensitivity to pain with anhidrosis (CIPA).
参考文献
Bossemeyer D. et al., 1989, J Biol Chem. 264: 16403-10.
Varsano T. et al., 2006 , Mol Cell Biol. 26: 8942-52.
Perrone L. et al., 2005, Biochem J. 385: 233-41.
Holtzman DM. et al., 2009 , J Neurosci. 15: 1567-76.
Dan C Lin. et al., 2006,Mol Cell Biol. 26: 8928-41.
Jung EJ. et al., 2008, Exp Mol Med. 40: 276-85.
规格：50ug
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