CD86 / B7-2蛋白产品信息
别名: CD86
表达方式:
A DNA sequence encoding the cynomolgus CD86 (Q9BDM4) (Met1-His239) was expressed with the Fc region of human IgG1 at the C-terminus.
种属: Cynomolgus
表达宿主: Human Cells
CD86 / B7-2 Protein QC Testing
纯度: (87.0+5.3)% as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Leu 20
分子量:
The recombinant cynomolgus CD86 is a disulfide-linked homodimer. The reduced monomer comprises 461 amino acids and has a calculated molecular mass of 52.2 KDa.The apparent molecular mass of the protein is approximately 76-86 and 34 KDa respectively in SDS-PAGE.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
CD86 / B7-2 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD86 / B7-2蛋白背景综述
CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte -associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. CD28 has two ligands expressed largely on professional APC, named B7-1 (CD80) and B7-2 (CD86). They are both type I transmembrane proteins with a membrane distal IgV and a membrane proximal Ig constant (IgC) domain and share 25% sequence identity. Although interacting with the same receptors, B7-1 and B7-2 show several distinct features. Binding of B7-2 with CD28 induces the activation of T cell, whereas CTLA-4 acts as a negative regulator and diminishes the immune responses. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
参考文献
Freeman GJ. et al., 1993, J Exp Med. 178 (6): 2185-92.
Mark DA. et al., 1998, Int Immunol. 10 (11): 1647-55.
Girndt M. et al., 2001, Kidney Int. 59 (4): 1382-9.
Tikkanen JM. et al., 2002, Am J Respir Crit Care Med. 165 (5): 724-9.
O'Neill SK. et al., 2007, J Immunol. 179 (8): 5109-16.
Salek-Ardakani S. et al., 2009, J Immunol. 82 (5): 1909-18.
规格：100ug
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