Cynomolgus Tie2 / TEK Protein|技术资料|现货

Tie2 / TEK蛋白产品信息

别名: TEK
表达方式: 
A DNA sequence encoding the cynomolgus TEK (G7PS68) (Met1-Lys745) was expressed with the Fc region of human IgG1 at the C-terminus.
种属: Cynomolgus
表达宿主: Human Cells

Tie2 / TEK Protein QC Testing

纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ala 23
分子量: 
The recombinant cynomolgus TEK is a disulfide-linked homodimer. The reduced monomer comprises 964 amino acids and has a calculated molecular mass of 107.6 KDa.The apparent molecular mass of the protein is approximately 63 KDa respectively in SDS-PAGE.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

Tie2 / TEK Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Tie2 / TEK蛋白背景综述

Tie2/Tek, together with Tie1/Tie, are members of the receptor tyrosine kinase (RTK) subfamily, and possess a unique extracellular domain containing two immunoglobulin-like loop domains flanking three epidermal growth factor (EGF) repeats followed by three fibronectin-type I?II repeats, and a split tyrosine kinase domain in the cytoplasmic region. Tie2 is expressed predominantly on endothelial and hematopoietic progenitor cells and is essential for blood vessel formation and maintenance. The identified ligand angiopoietin-1 (Ang-1) binds Tie2 receptor with high-affinity and induces the signaling pathway of both cell migration and cell survival via the related mediator such as GRB2 and SH-PTP2. Accordingly, the Tie2 may trigger crucial endothelial responses during embryonic vascular development and during pathologic vascular growth, as well as the inflammatory responses with the key regulator ABIN-2. Recent study has revealed that Ang1-Tie2 signaling exerts different function at cell-cell contacts and cell-substratum contacts dependent upon the spatial localization of Tie2. Deficiency in this gene is associated with inherited venous malformations.

参考文献

Partanen J. et al., 1992, Mol Cell Biol. 12: 1698-707.
Sato TN. et al., 1993, Proc Natl Acad Sci. 90: 9355-8.
Jones N. et al., 1999, J Biol Chem. 274: 30896-905.
Fukuhara S. et al., 2008, Nat Cell Biol. 10: 513-26.
Marron MB. et al., 2007, J Biol Chem. 282: 30509-17.
Huqhes DP. et al., 2003, Circ Res. 92: 630-6.

规格：100ug

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