Siglec-2 / CD22 (aa 176-687) Protein|蛋白质|现货

Siglec-2 / CD22蛋白产品信息

别名: RP11-446H13.1, FLJ41758, FLJ43475, FLJ44957
表达方式: 
A DNA sequence encoding the human CD22 (Q32M46) (Trp176-Arg687) was fused with a polyhistidine tag at the C-terminus.
种属: Human
表达宿主: Human Cells

Siglec-2 / CD22 Protein QC Testing

纯度: > 90 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Trp 176
分子量: 
The recombinant human CD22 consists of 523 amino acids and predictes a molecular mass of 58.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant protein is approximately 90-100 kDa due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

Siglec-2 / CD22 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Siglec-2 / CD22蛋白背景综述

CD22 is a molecule belonging to the immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family. It is a sugar binding transmembrane protein, which specifically binds sialic acid with an immunoglobulin (Ig) domain located at its N-terminus. The CD22 cell-surface adhesion molecule is capable of modulating B lymphocyte antigen receptor (BCR)-mediated signals, as well as the generation of BCR-independent signals. CD22 is an inhibitory coreceptor of the B-cell receptor (BCR), and plays a critical role in establishing signalling thresholds for B-cell activation. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. Interaction of CD22 to ligands in trans can regulate both B-cell migration as well as the BCR signaling threshold. However, a more complex role for CD22 has recently emerged, including a central role in a novel regulatory loop controlling the CD19/CD21-Src-family protein tyrosine kinase (PTK) amplification pathway that regulates basal signaling thresholds and intensifies Src-family kinase activation after BCR ligation. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1. Thus, the CD19/CD22 loop may be a potential therapeutic target in autoimmune disease for modulating B cell signaling.

参考文献

Sato S. et al., 1998, Semin Immunol. 10 (4): 287-97.
Walker JA. et al., 2008, Immunology. 123 (3): 314-25.
Nitschke L. 2009, Immunol Rev. 230 (1): 128-43.
Tedder TF. et al., 2005, Adv Immunol. 88: 1-50.
Fujimoto M. et al., 2007, J Dermatol Sci. 46 (1): 1-9.

规格；50ug

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