c-MET / HGFR蛋白产品信息
别名: MET, Capza2, Cav , Cav2 , Cftr , LOC500040 , ST7 , Wnt2
表达方式:
A DNA sequence encoding the rat MET (Q2IBC7) (Met1-Asn932) was expressed with the Fc region of human IgG1 at the C-terminus.
种属: Rat
表达宿主: Human Cells
c-MET / HGFR Protein QC Testing
纯度: (63.0+32.1)% as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Glu 25
分子量:
The recombinant rat MET/Fc is a disulfide-linked homodimer. The reduced monomer comprises 1149 amino acids and has a predicted molecular mass of 128.1 kDa. The apparent molecular mass of the protein is approximately 97 and 39 kDa in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
c-MET / HGFR Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
c-MET / HGFR蛋白背景综述
The proto-oncogene MET encoded product MET (mesenchymal-epithelial transition factor), also known as c-Met or hepatocyte growth factor receptor (HGFR), is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. As the prototypic member of a small subfamily of growth factor receptors, c-Met/HGFR is synthesized as a single chain precursor, and is processed into a mature disulfide-linked heterodimer composed of a extracellular α subunit and a transmembrane β subunit via post-translational cleavage. c-Met/HGFR is identified as a glycosylated receptor tyrosine kinase (RTK), and HGF is the only known ligand. Following ligand binding and autophosphorylation, c-Met/HGFR transmits intercellular signals using a unique multisubstrate docking site which mediates the binding of multiple SH2-containing adapter proteins such as Grb2, SHC, Crk/CRKL, as well as Gab1. Normal c-Met/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active c-Met/HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. Two transcript variants encoding different isoforms have been identified.
参考文献
Bottaro DP. et al., 1991, Science. 251: 802-4.
Birchmeier C. et al., 2003, Nat Rev Mol Cell Biol. 4: 915-25.
Weidner KM. et al., 1996, Nature. 384: 173-6.
Pennacchietti S. et al., 2003, Cancer Cell. 3: 347-61.
Boccaccio C. et al., 2006, Nat Rev Cancer. 6: 637-45.
Chmielowiec J. et al., 2007, J Cell Biol. 177: 151-62.
Yu YL. et al., 2002, Cancer Res. 62: 2951-6.
Furge KA. et al., 2000, Oncogene. 19: 5582-9.
规格：50ug
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