PDGFRB / PDGFR-1蛋白产品信息
别名: PDGFRB
表达方式:
A DNA sequence encoding the cynomolgus PDGFRB (F7BTH1) (Met1-Phe530) was expressed with a polyhistidine tag at the C-terminus.
种属: Cynomolgus
表达宿主: Human Cells
PDGFRB / PDGFR-1 Protein QC Testing
纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Leu 33
分子量:
The recombinant cynomolgus PDGFRB comprises 509 amino acids and has a calculated molecular mass of 57.7 KDa. The apparent molecular mass of it is approximately 80-90 KDa respectively in SDS-PAGE.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
PDGFRB / PDGFR-1 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PDGFRB / PDGFR-1蛋白背景综述
Beta-type platelet-derived growth factor receptor, also known as CD140b, PDGFRB, PDGF-R-beta, PDGF receptor-β, is a member of the protein kinase superfamily and CSF-1/PDGF receptor subfamily. The PDGF family consists of PDGF-A, -B, -C and -D, which form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-α and -β. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -αα, -ββ and -αβ. The extracellular?region of the PDGF receptor-β consists of five?immunoglobulin-like domains while the intracellular part is a?tyrosine kinase domain. The ligand-binding sites of PDGF receptor-α and -β are located to the three first immunoglobulin-like domains. PDGF-CC specifically interacts with PDGFR-αα and -αβ, but not with –ββ, and thereby resembles PDGF-AB. PDGF-DD binds to PDGFR-ββ with high affinity, and to PDGFR-αβ to a much lower extent and is regarded as PDGFR-ββ specific. PDGF-AA binds only to PDGFR-αα, while PDGF-BB is the only PDGF that can bind all three receptor combinations with high affinity.
参考文献
Chi KD. et al., 1997, Oncogene. 15: 1051-7.
Arcaro A. et al., 2000, Mol Cell Biol. 20: 3817-30.
Bergsten E. et al., 2001, Nat Cell Biol. 3: 512-16.
Zhang Z. et al., 2004, Protein Sci. 13: 2819-24.
Morerio C. et al., 2004, Cancer Res. 64: 2649-51.
规格：100ug
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