Cynomolgus / Rhesus B7-H1 / CD274 / PD-L1 Protein|报价|使用说明

B7-H1 / CD274 / PD-L1蛋白产品信息

别名: CD274
表达方式: 
A DNA sequence encoding the cynomolgus/rhesus CD274 (F6VEW6) (Met1-Thr239) was expressed with a polyhistidine tag at the C-terminus. Cynomolgus and Rhesus CD274 sequences are identical.
种属: Cynomolgus
表达宿主: Human Cells

B7-H1 / CD274 / PD-L1 Protein QC Testing

纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Phe 19
分子量: 
The recombinant cynomolgus/rhesus CD274 comprises 232 amino acids and has a calculated molecular mass of 26.7 KDa. The apparent molecular mass of it is approximately 37 KDa in SDS-PAGE.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

B7-H1 / CD274 / PD-L1 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

B7-H1 / CD274 / PD-L1蛋白背景综述

B7-H1, also known as CD274 and PD-L1, is a cell surface glycoprotein belonging to the B7 family of costimulatory molecules. B7-H1 is originally identified by homology analysis in comparison with B7-1 and B7-2, two molecules with important immunoregulatory functions. B7-H1 costimulates T-cell proliferation and preferentially induces IL-10 and IFN-gamma secretion in a PDCD1-independent manner. The costimulatory function of B7-H1 is critical for enhancing maturation and differentiation of T-cells in lymphoid organs. On contrast, B7-H1 also functions as a co-inhibitor through programmed death-1 (PD-1). By binding to PD-1 receptors on activated T-cells and B-cells, B7-H1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production, and thus inhibits ongoing T-cell responses in peripheral tissues. Aberrantly expressed of B7-H1 by tumor cells or lymphocytes is associated with aggressive pathologic features of certain carcinomas and represents a promising target for immune manipulation. Mouse and human B7-H1/PD-L1 share 73% amino acid sequence identity.

参考文献

Petrella J. et al., 2002, J Virol. 76 (20): 10503-6.
Tamura H. et al., 2003, Int J Hematol. 78 (4): 321-8.
Holets L M. et al., 2006, Biol Reprod. 74 (2):352-8.
Thompson R H. et al., 2006, Clin Genitourin Cancer. 5 (3): 206-11.

规格：100ug

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