IL23A & IL12B Heterodimer蛋白产品信息
别名: IL23A & IL12B
表达方式:
A DNA sequence encoding the marmoset IL23A (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the marmoset IL12B (Met 1-Asn 328) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the marmoset IL23A & the marmoset IL12B heterodimer was purified.
种属: Marmoset
表达宿主: Human Cells
IL23A & IL12B Heterodimer Protein QC Testing
纯度: > 85 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Arg 20 & Ile 23
分子量:
The recombinant marmoset IL23A & the marmoset IL12B comprises 498(181+317) amino acids and has a calculated molecular mass of 56.4 (20.1+36.3) KDa. The apparent molecular mass of the marmoset IL23A & the marmoset IL12B heterodimer is approximately 22 & 45 KDa respectively in SDS-PAGE.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
IL23A & IL12B Heterodimer Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL23A & IL12B Heterodimer蛋白背景综述
Interleukin-23 subunit alpha, also known as IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19 and IL23A, is a secreted protein which belongs to the?IL-6 superfamily. IL-23A associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Interleukin-12 subunit beta, also known as cytotoxic lymphocyte maturation factor 40 kDa subunit, NK cell stimulatory factor chain 2, NKSF2 and IL12B, is secreted protein which belongs to the?type I cytokine receptor family and Type 3 subfamily. IL12B / NKSF2 is a ctokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. IL12B / NKSF2 associates with IL-23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues.
参考文献
Altare F. et al., 1998, J Clin Invest. 102: 2035-40.
Oppmann B. et al., 2000, Immunity. 13: 715-25.
Picard C. et al., 2002, Am J Hum Genet. 70: 336-48.
Parham C. et al., 2002, J Immunol. 168: 5699-708.
Pirhonen J. et al., 2002, J Immunol. 169: 5673-8.
规格：10ug
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