VEGF / VEGFA蛋白产品信息
别名: VEGFA, VEGF
表达方式:
A DNA sequence encoding the canine VEGFA(NP_001103972.1) (Met1-Arg190) was expressed.
种属: Canine
表达宿主: Human Cells
VEGF / VEGFA Protein QC Testing
纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ala 27
分子量:
The recombinant canine VEGFA comprises 164 amino acids and has a predicted molecular mass of 19.1 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
VEGF / VEGFA Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
VEGF / VEGFA蛋白背景综述
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and *****. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. As its name implies, VEGF-A activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (stimulation monocyte/macrophage, neurons, cancer cells, kidney epithelial cells ). VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Alternatively spliced transcript variants of VEGF-A, encoding either secreted or cell-associated isoforms, have been identified. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform and is a heparin-binding growth factor with mitogenic activity for vascular endothelial cells. Human VEGF165 shares 88%, 96%, 95% and 93% aa sequence identity with mouse, porcine, canine and feline VEGF165, respectively.
参考文献
Mattei MG. et al., 1996, Genomics. 32 (1):168-9.
Borgatti P. et al.,1998, Eur J Immunol. 27 (11): 2805-11.
Gengrinovitch S. et al., 1999, J Biol Chem. 274 (16): 10816-22.
Zachary I. et al., 2001, Cardiovasc. Res. 49: 568-81.
Robinson CJ. et al., 2001, J Cell Sci. 114: 853-65.
Lee P. et al., 2002, Proc Natl Acad Sci. 99: 10470-5.
Lee GY. et al., 2006, Protein expressed and Purification. 46: 503-9.
规格：10ug
关 键 词：Canine VEGF / VEGFA Protein,技术资料,现货，实验室耗材，生化试剂，上海创赛，博飞美科，实验仪器，厂家
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