GFRA1 / GFR alpha-1蛋白产品信息
别名: GFRA1, Gdnfra, Retl1, Trnr1
表达方式:
A DNA sequence encoding the rat GFRA1 (Q62997) (Met1-Leu445) was expressed with the Fc region of human IgG1 at the C-terminus.
种属: Rat
表达宿主: Human Cells
GFRA1 / GFR alpha-1 Protein QC Testing
纯度: > 90 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Asp 25
分子量:
The recombinant rat GFRA1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 662 amino acids and has a predicted molecular mass of 73.8 kDa. The apparent molecular mass of the protein is approximately 85 kDa in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
GFRA1 / GFR alpha-1 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
GFRA1 / GFR alpha-1蛋白背景综述
GFR alpha -1 is a novel glycosylphosphatidylinositol (GPI)-linked cell surface receptor, and belongs to the glial cell line-derived neurotrophic factor (GDNF) receptor subfamily consisting of at least four members termed GFR alpha 1-4. The GDNF family ligands comprises GDNF, Neurturin (NTN), Artemin, and Persephin, and upon the specifically GDNF binding with high affinity, GFRα-1 mediates the association with and activation of the RET tyrosine kinase receptor (RTK), and subsequently initiates the RET signaling pathway. GFR alpha-mediated signaling plays an important role in the survival, differentiation, and migration of central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system, as well as the inflammatory response in some carciniomas. In addition, recent studies have revealed that GFRα-1 also associates with the neural cell adhesion molecule NCAM and promotes high affinity GDNF binding to NCAM, and accordingly down-regulates NCAM-mediated homophilic cell adhesion via the RET-independent signaling.
参考文献
Jing S. et al., 1996, Cell. 85: 1113-24.
Treanor JJ. et al., 1996, Nature. 382: 80-3.
Paratcha G. et al., 2003, Cell. 113: 867-79.
Esseqhir S. et al., 2007, Cancer Res. 67: 11732-41.
Sjostrand D. et al., 2008, J Biol Chem. 283: 13792-8.
Lee RH. et al., 2006, J Neurosci. Res. 83: 80-90.
规格：50ug
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