CD3D & CD3E Heterodimer蛋白产品信息
别名: CD3D & CD3E
表达方式:
A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
种属: Human
表达宿主: Human Cells
CD3D & CD3E Heterodimer Protein QC Testing
纯度: (43.8+13.1+41.9) % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Phe 22 & Asp 23
分子量:
The recombinant heterodimer of human CD3D&CD3E comprises 272 (126+146) amino acids and has a calculated molecular mass of 31.2 (14.4+16.8)KDa. As a result of glycosylation, the apparent molecular mass of human CD3D&CD3E heterodimer is approximately 20, 22 and 26 KDa in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
CD3D & CD3E Heterodimer Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD3D & CD3E Heterodimer蛋白背景综述
T-cell surface glycoprotein CD3 delta/epsilon chain, also known as CD3D and CD3E respectively, are single-pass type I membrane proteins. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). The immunofluorescence defined that CD3 epsilon(CD3E0) was strongly stained bound to either CD 3 gamma or CD 3 delta. The recognition by this panel of anti-CD3 antibodies of CD3-gamma/ epsilon and CD3-delta/ epsilon complexes and not of CD3-epsilon alone was assessed by immunoprecipitation.
参考文献
Wong S. et al., 1991, Clin Exp Immunol. 83 (1): 69-73.
Call ME. et al., 2004, EMBO J. 23 (12): 2348-57.
Arnett KL. et al., 2004, Proc Natl Acad Sci. 101: 16268-73.
Kjer-Nielsen L. et al., 2004, Proc Natl Acad Sci. 101: 7675-80.
Fischer A. et al., 2005, Curr Opin Allergy Clin Immunol. 5 (6): 491-5.
Holst J. et al., 2008, Nature immunology. 9 (6): 658-66.
Salmerón A. et al., 1991, J Immunol. 147 (9): 3047-52.5.
规格：20ug
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