ATP1B1 Protein|ATP1B1蛋白质|使用说明|产品详情

ATP1B1蛋白产品信息

别名: ATP1B1, ATP1B
表达方式: 
A DNA sequence encoding the human ATP1B1 (P05026-1) (Glu63-Ser303) was expressed with an N-terminal polyhistidine tag.
种属: Human
表达宿主: Human Cells

ATP1B1 Protein QC Testing

纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: His
分子量: 
The recombinant human ATP1B1 comprises 261 amino acids and has a predicted molecular mass of 30.4 kDa. The apparent molecular mass of the protein is approximately 40-47 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

ATP1B1 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ATP1B1蛋白背景综述

ATP1B1 belongs to the family of Na+/K+ and H+/K+-ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. ATP1B1 is a subunit of Na+/K+-ATPase. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. Na+/K+-ATPase is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). ATP1B1 regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. ATP1B1 is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane.

参考文献

Lingrel JB. et al., 1990, Prog Nucleic Acid Res Mol Biol. 38: 37-89.
Oakey RJ. et al., 1993, Hum Mol Genet. 1 (8): 613-20.
Ushkaryov YuA. et al., 1990, FEBS Lett. 257 (2): 439-42.

规格：50ug

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