H7N9 (A/Shanghai/1/2013) 血凝素(HA1 / Hemagglutinin) 蛋白 Product Information
别名: HA1
表达方式:
A DNA sequence encoding the Influenza A virus (A/Shanghai/1/2013(H7N9)) hemagglutinin (Met1-Gly338), termed as HA1, was expressed with a C-terminal polyhistidine tag.
种属: Influenza A virus
表达宿主: Human Cells
H7N9 (A/Shanghai/1/2013) 血凝素(HA1 / Hemagglutinin) 蛋白 QC Testing
纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Asp 19
分子量:
The recombinant HA1 subunit of Influenza A virus (A/Shanghai/1/2013(H7N9)) hemagglutin comprises 331 amino acids and has a predicted molecular mass of 36.3 kDa. The apparent molecular mass of the protein is approximately 42-47 kDa in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
H7N9 (A/Shanghai/1/2013) 血凝素(HA1 / Hemagglutinin) 氨基酸序列
DKICLGHHAV SNGTKVNTLT ERGVEVVNAT ETVERTNIPR ICSKGKRTVD LGQCGLLGTI TGPPQCDQFL EFSADLIIER REGSDVCYPG KFVNEEALRQ ILRESGGIDK EAMGFTYSGI RTNGATSSCR RSGSSFYAEM KWLLSNTDNA AFPQMTKSYK NTRKNPALIV WGIHHSGSTA EQTKLYGSGN KLVTVGSSNY QQSFVPSPGA RTQVNGQSGR IDFHWLMLNP NDTVTFSFNG AFIAPDRASF LRGKSMGIQS GVQVDADCEG DCYYSGGTII SNLPFQNIDS RAVGKCPRYV KQRSLLLATG MKNVPEIPKG AHHHHHHHHH H
H7N9 (A/Shanghai/1/2013) 血凝素(HA1 / Hemagglutinin) 蛋白 Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
H7N9 (A/Shanghai/1/2013) 血凝素(HA1 / Hemagglutinin) 蛋白描述
H7N9 is a subtype of Influenza virus A. On April 1, 2013, the World Health Organization (WHO) first reported 3 human infections with a new influenza A (H7N9) virus in China. Since then, additional cases have been reported. This new H7N9 virus is an avian (bird) influenza (flu) virus. Influenza (flu) is a respiratory infection in mammals and birds. The virus is divided into three main types (Influenza A, Influenza B, and Influenza C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA).
Influenza A is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. The notation HhNn is used to refer to the subtype comprising the hth discovered Hemagglutinin (HA) protein and the nth discovered neuraminidase (NA) protein. The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.
The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
参考文献
Barman S. et al., 2000, J Virol. 74: 6538-45.
Suzuki T. et al., 2005, J Virol. 79: 11705-15.
Shinya K. et al., 2006, Nature. 440 (7083): 435-6.
Marjuki H. et al., 2006, J Biol Chem. 281: 16707-15.
Christophe F. et al., 2009, Science. 324: 1557-61.
Von Itzstein M. 2007, Nat Rev Drug Discov. 6: 967-74.
规格：50ug
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