DNASE1 / Deoxyribonuclease I / DNL1 Protein|使用说明|现货

DNASE1 / Deoxyribonuclease I / DNL1蛋白产品信息

别名: DNASE1, DNL1, DRNI
表达方式: 
A DNA sequence encoding the human DNASE1 (P24855) (Met1-Lys282) was expressed with a polyhistidine tag at the C-terminus.
种属: Human
表达宿主: Human Cells

DNASE1 / Deoxyribonuclease I / DNL1 Protein QC Testing

纯度: > 85 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Leu 23
分子量: 
The recombinant human DNASE1 consists of  271 amino acids and predicts a molecular mass of  30.7 KDa. It migrates as an approximately 37 KDa band in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

DNASE1 / Deoxyribonuclease I / DNL1 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

DNASE1 / Deoxyribonuclease I / DNL1蛋白背景综述

DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.

参考文献

Shak S. et al., 1991, Proc Natl Acad Sci. 87 (23): 9188-92.
Yasutomo K. et al., 2001, Nat Genet. 28 (4): 313-4.
Hakkim A. et al., 2010, Proc Natl Acad Sci. 107 (21): 9813-8.

规格：10ug

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