MMGT1 / EMC5 Protein|MMGT1 / EMC5蛋白质|使用说明|现货

MMGT1 / EMC5蛋白产品信息

别名: MMGT1, EMC5 TMEM32
表达方式: 
A DNA sequence encoding the human MMGT1 (NP_775741.1) (Glu66-Arg131) was expressed with an N-terminal polyhistidine tag.
种属: Human
表达宿主: Human Cells

MMGT1 / EMC5 Protein QC Testing

纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: His
分子量: 
The recombinant human MMGT1 comprises 86 amino acids and has a predicted molecular mass of 10.1 kDa. The apparent molecular mass of the protein is approximately 23-29 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

MMGT1 / EMC5 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MMGT1 / EMC5蛋白背景综述

MMGT1, also known as EMC5, is comparable with primary microglial cells with respect to morphology, presence of acetylated low density lipoprotein receptor, non-specific esterase, CD63, major histocompatibility complex antigens and CD11, and binding for Ricinus communis agglutinin. Primary microglia as well as MMGT1 cells are negative for glial fibrillary acidic protein. Different MMGT1 strains are obtained after subcloning, two of which resembled histiocytes (F4/80 and BM-8). These cell strains, MMGT12 and 16, are able to opsonize latex beads, and could be induced by endotoxins (LPS) to secrete TNF-alpha, IL-1, IL-6, TGF-beta, and EGF.

参考文献

Goytain A. et al., 2008, Am J Physiol Cell Physiol. 294 (2): C495-502.
Briers TW. et al., 1994, J Neuroimmunol. 52 (2): 153-64.
Jäger S. et al., 2011, Nature. 481 (7381): 365-70.

规格：50ug

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